Kamis, 10 Maret 2011

Microbiology Laboratory Equipment


 MICROBIOLOGICAL LABORATORY EQUIPMENT

In this blog contains spesifications for microbiological laboratory equipment.
1.          Incubators
Incubators must maintain a uniform and constant temperature at all times in all areas, that is, they must not vary more than + 0,5°C in the areas used.  Obtain such accuracy by using a water-jacketed or anhydric-type incubator with thermostatically controlled  low-temperature electric heating units properly insulated and located or adjacent to the walls or floor of the chamber and preferably equipped with mechanical means of circulating air.
Incubator equipped with high-temperature heating units are unsatisfactory, because such sources of heat, when inproperly placed, frequenyly cause localized overheating and excessive drying of media, with consequent inhibition of bacterial growth.
2.         Hot-Air Sterilizing oven
Used hot air sterilizing oven of sufficient size to prevent internal crowding ; constructed to give uniform and adequate sterilizing temperature of 170 + 10°C ; and equipped with suitable thermometers.  Optionally use a temperature-recording instrument.
3.         Autoclaves
Use autoclaves of sufficient size to prevent internal crowding ; constructed to provide uniform temperature within the chamber (up to and including the sterilizing temperature of 121°C):equipped with an accurate thermometer the bulb of which is locatedproperly of the exhaust line so as to register minimum temperature within the sterilizing chambers(temperature-recording instruments is optional) ; equipped with pressure gauge and properly adjusted safety valves connected directly with saturated steam supply line equipped or appropriate filters to remove particulates and iol droplets or directly to a suitable special steam generator (do not use steam from boiler treated with animes for corrosion control) ; and capable of reaching desired temperature within 30 minute.
Comfirm, by chemical or toxicity tests, that the steam supply has not been treated with amines or other corrosion-control chemicals that will impart toxicity.
4.         Balances
Use balances providing a sensitvity of at least 0,1 g at a load of 150 g, with appropriate weights.  Use an analitycal balance having a sensitivity of 1 mg under a load of 10 g for weighing small quantities (less than 2 g) of materials.  Single-pan rapid-weigh balances are most convenient.


5.         Media Preperation Utensils.
Use borosilicate glass or other suitable noncorrosive equipment such as stainless steel.  Use glassware that is clean and free of residues, dried agar or other foreign materials that may contamined media.
6.         Pipet and Graduated Cylinders.
Use pipets of any convinent size, provided that they deliver that required volume accurately and quickly.  The error of calibration  for a  given  manufacturer’s lot must not exceed 2,5%.
Use pipets having graduations distinctly marked and with unbroken tips.  Bacteriological transfer pipets or pipet conforming to APHA standards may be used.  Do not pipet by mouth ; use pipets aid.
Use graduated cylinders meeting ASTM Standard (D-86 and D-216) and with accuracy limits established by NIST where appropriate.
7.         Pipet Container
Use of boxes alumunium or stainless steel, end measurement 5 to 7,5 cm, cylindrical or rectangular, and length about 40 cm.  When these are not available,, paper warppings for individual pipet may be subsituted.  To avoid excessive charring during sterilization, use best-quality sulfate pulp (kraft) paper.  Do not use copper or copper alloy cans or boxes as pipet containers.  
8.         Refriagerator
Use a refrigerator maintaining a temperature of 1 to 4.4 °C  to store sample, media, reagent, etc.  Do not store volatile solvents, food, or beverages in a refrigerator with media.  Frost-free refrigerators may cause excessive media dehydration on storage longer than 1 week.
9.         Petri Dishes
For the plate count, use glass or plstic petri dishes about 100x15mm.  Use dishes the bottoms of which are free from bubbles and scratches and flat so that the medium will be of uniform thickness throughout the plate.  For the membran filter technique use loose-lid glass or plastic dishes, 60x15 mm or tight-lid  50x12 mm.  Sterilize petri dishes and store in metal cans (alumunium or stainless steel but not copper) or wrap in paper-preferably best-quality sulfate pulp (kraft)-before sterilizing.  Presterilized petri dishes are commercially available.
10.     Membrane Filtration Equipment
Use filter funnel and membrane holder made of seamless stainless steel, glass, or autoclavable plastic that does not leak and is not subject to corrosion.  Field laboratory kits are acceptable but standard laboratory filtration equipment and procedures are required.

11.     Fermentation Tube and Vial
Use fermentation tubes of any type, if their design permits conforming to medium and volume requirements for concentration of nutritive ingredients as discribed subsequently.  Where tubes used for a test of gas production, enlose a shell vial, inverted.
Use tubes and vial of such size that the vial will be filled completely with medium, at least partly submerged in the tube, and large enough to make glass bubbles easily visible.
12.     Sample Bottles
For bacteriological samples, use sterilizable bottle of glass or plastic of any suitable size and shape.  Use bottle capable of holding a sufficient volume of sample for all required test and an adequite air space, premitting proper washing and maintaining sample uncontaminated until examinations are complete.   Ground-glass-stoppered bottles, preferably wide-mounthed and of resostant glass, are recommanded.  Plastic bottles of suitable size, wide-mouthed, and made of nontoxic materials such as polyprpylene than can be sterilized repeatedly are satisfactory as sample container.
Before sterilization, cover tops and necks of sample bottles having glass closures with alumunium foil or heavy kraft paper.

49 komentar:

  1. waaaaah ....ilmu yang sangat bermanfaat

    BalasHapus
  2. Hola,espero que tengas un traductor para poder entenderme,gracias por hacerte seguidor de mi blog.

    Saludos y hasta pronto.

    BalasHapus
  3. remember having to use all of these when I had taken Biology!!!

    BalasHapus
  4. Haha this brings back scary memories of Biology class... ;)


    xoxo,
    Addie
    The Cat Hag

    BalasHapus
  5. Great blog! Thanks for dropping by, new follower here :)
    diane
    http://whatsrealityallabout.blogspot.com

    BalasHapus
  6. thanks for comming bye, I really appreciate it!

    x Nura from 'World of Fashion Inspiration'

    BalasHapus
  7. Your from Indonesia? I love this country! Especially Sumatra is absolutely beautiful!

    http://ryf-style.blogspot.com

    BalasHapus
  8. Hi there. Nice blog. You have shared useful information. Keep up the good work! This blog is really interesting and gives good details. steam sterilizer, autoclaves.

    BalasHapus
  9. Muy interesante la información, instructivo.Fue un gusto leerte y visitarte. Seguiré visitándote. Que tengas un buen día Mengenai. Te envío un cálido saludo.

    http://socialculturalyhumano.blogspot.com/

    BalasHapus
  10. Interesting blog! keep up the good work! I follow you as well

    BalasHapus
  11. it looks super interesting!!
    very nice blog!! i'm following you by google friends box hope you'll do the same if you'll like mine :)
    xoxo

    Syriously in Fashion
    Facebook

    BalasHapus
  12. MUITO BOM! Eu acredito que Deus criou o homem e deixou o manual de instrução, a “BÍBLIA SAGRADA!” A bíblia é a divina revelação da palavra de Deus e não produtos enlatados, eu costumo dizer que: “As pessoas precisam parar de comer tudo o que dão. E começar a questionar! ” Alguém escuta alguma coisa de alguém, e saem falando, sem questionar, sem saber se é verdade ou não. As pessoas precisam se perguntar! Será que a minha crença esta de acordo com a lei de Deus? Será que a religião que eu nasci sempre me falou a verdade? Quando uma pessoa morre pra onde ela vai? Ela volta? A bíblia apóia o homossexualismo? Quem não herdara o reino de Deus? O que devo saber sobre idolatria, imagens e escultura? O que devo saber sobre o único batismo? Será que tem uma seqüência para salvação? A quem eu devo me confessar, quem pode me perdoar? Religião salva alguém? Essas e outras perguntas e respostas em:
    www.aunicaverdadeemsuapropriabiblia.blogspot.com
    Você pode dizer, eu já sou salvo amém! Vamos atrás de quem não esta, divulgue este site, porque: “Eis que vêm dias, diz o Senhor Jeová, em que enviarei fome sobre a terra, não fome de pão, nem sede de água, mas de ouvir as palavras do Senhor. E irão vagabundos de um mar até outro mar, e do norte até ao oriente; correrão por toda parte, buscando a palavra do Senhor, e não a acharão." ( Amós cap 8 ver 11 e 12 )Fale para outras pessoas, por que “O Senhor não retarda a sua promessa, ainda que alguns têm por tardia: mas é longânimo para convosco, não querendo que alguns se percam, senão que todos venham a arrepender-se.” (II Pedro cap. 3 ver 8) e “Que quer que todos os homens se salvem, e venham ao conhecimento da verdade. Porque há um só Deus, e um só Mediador entre Deus e os homens, Jesus Cristo o homem. (I A Timóteo cap 2 ver 5 e 6)
    Seja sua bíblia, católica ou evangélica, aqui você vai tirar suas duvidas.
    www.aunicaverdadeemsuapropriabiblia.blogspot.com
    E conhecereis a verdade, e a verdade vos libertara (João cap 8 ver 32) Se você tiver coragem de perguntar, a bíblia terá coragem de responder!

    BalasHapus
  13. ...traigo
    sangre
    de
    la
    tarde
    herida
    en
    la
    mano
    y
    una
    vela
    de
    mi
    corazón
    para
    invitarte
    y
    darte
    este
    alma
    que
    viene
    para
    compartir
    contigo
    tu
    bello
    blog
    con
    un
    ramillete
    de
    oro
    y
    claveles
    dentro...


    desde mis
    HORAS ROTAS
    Y AULA DE PAZ


    COMPARTIENDO ILUSION
    MICTOBIOLOGY

    CON saludos de la luna al
    reflejarse en el mar de la
    poesía...




    ESPERO SEAN DE VUESTRO AGRADO EL POST POETIZADO DE STAR WARS, CARROS DE FUEGO, MEMORIAS DE AFRICA , CHAPLIN MONOCULO NOMBRE DE LA ROSA, ALBATROS GLADIATOR, ACEBO CUMBRES BORRASCOSAS, ENEMIGO A LAS PUERTAS, CACHORRO, FANTASMA DE LA OPERA, BLADE RUUNER ,CHOCOLATE Y CREPUSCULO 1 Y2.

    José
    Ramón...

    BalasHapus
  14. hola!!!! navegando por la red ,entre blog y blog ,me he encontrad con el tuyo!!!! decirte que me ha encantada ,ya me he hecho tu seguidora , me encanta encontrar rincones nuevos , propuestas y estilos diferentes y como no, apoyaros a cada una!!!
    os dejo el mio también!!!! si te gusta también agradecería tu apoyo todas sabemos lo que cuesta tener un blog y es bonito el apoyo entre nosotras!!! si no nos apoyamos , quien lo va a hacer¿?¿?¿ un besote de " KEYKOAMODEL.BLOGSPOT.COM"

    BalasHapus
  15. blog interresante e bem montado, estou passando
    e lendo suas escritas... boa mesmo. Abraço.

    BalasHapus
  16. Oi,amiguinho(achei legal seu blog),visite/siga o Blog do XANDRO(meu blog)vc vai gostar!;)

    http://blogdoxandro.blogspot.com/

    BalasHapus
  17. Hello! Thank you happy.
    I am looking forward to your blog.
    I was really hard! Sentence.

    mukup!

    BalasHapus
  18. Saludos:
    Los seres que iniciaron todo un mundo de vida, que nos rodean en silencio, que son nuestros asesinos o nuestros salvadores. Un blog interesante. Gracias.

    BalasHapus
  19. Very informative, thanks for sharing.

    BalasHapus
  20. Des informations très précises... Je découvre votre blog.
    Bisous

    BalasHapus
  21. Nice blog! (--:

    Mine:
    www.with-love-juulia.blogspot.com

    BalasHapus
  22. very good information !

    ellanjaamandan.blogspot.com

    BalasHapus
  23. Amigo;
    Deixar para trás,
    o que não nos importou,
    o que não nos importa mais

    Viver e acreditar,
    almejar e sonhar,
    se dar um pouco de sí,
    viver sem medo de sorrir

    Cultivar a esperança,
    de peitos abertos a ela se entregar,
    ter a alegria de uma criança,
    e nos rios da vida navegar

    E assim prosseguir, viver,
    sem medo de perder ou ganhar,
    pois a vida só faz valer,
    para aqueles que tem coragem de sonhar.

    Beijão da amiga Cátia
    http://catiaartesmanuais.blogspot.com/

    BalasHapus
  24. спасибо,что вы побывали у меня в гостях! гостей я всегда люблю и приветствую!заходите чаще,я всегда вам буду рада!я тоже буду к вам наведоваться,у вас тут интерессный материал для людей всего мира!

    BalasHapus
  25. Gracias por visitar mi sitio, y ser mi seguidor. Un cordial abrazo desde Argentina. Te sigo también.

    BalasHapus
  26. Wow, This is great information! :) I am a student of biology! :) Check out my blog!

    BalasHapus
  27. hope you like my blog...
    showroomdegarde.blogspot.com

    kisses
    fran

    BalasHapus
  28. Nice Blog ! ; - )

    Mine :
    http://niusiaa.blogspot.com/

    BalasHapus
  29. Hey meine Liebe :) Ich habe mir einen neuen erstellt und werde meinen alten Blog in kürzester Zeit auflösen. Ich wollte einfach noch mal einen „Neubeginn“ starten. Würde mich freuen wenn du mir trotzdem als Leser treu bleibst und mich auch weiterhin verfolgst. So wie ich dich natürlich auch. Liebste Grüße, Jenny ♥ http://weallhaveserects.blogspot.de/

    BalasHapus
  30. Dear friend, have a nice month!
    Anthonis

    BalasHapus
  31. hi all my friends,just support me for the contest of migrant worker solidarity, on http://kontesblog.buruhmigran.or.id/?p=75 with all my respect may GOD bless u all thanks and have a nice day

    BalasHapus
  32. nice blog..follow and support you

    BalasHapus
  33. interesting post :) greets from Poland xXx

    BalasHapus
  34. Hi there! Just stopped by to introduce myself and my blog:) I post almost everyday from Japan about high and low fashion. I love fashion, and introduce Japanese fashion, snap shots, and many more. Hopefully you come by and follow if you like it. Thanks for taking your time to read! Thanks again;)

    Little K.
    http://lilkfashionista.blogspot.jp/

    BalasHapus
  35. Hooooola! Preciosa entrada y un blog muy chulo. Te sigo sin dudar y por si quieres visitar mis blogs y dejar una marca en ellos te los dejo aquí ;)

    http://myflashdreams.blogspot.com/

    (Blog donde publico fotografías echas por mi y escribo sobre mi manera de ver las cosas. Pronto le cambiaré el título y la dirección web pero con un mensaje de antelación en una entrada)

    http://myimpposiblesarecomingtrue.blogspot.com.es/

    (Blog más romántico con fotografías igualmente echas por mi, animaciones gif, etc )

    BalasHapus
  36. The PCBs we assemble comply with standards adopted by ISO 9001-2000, as well as any other requirements that are relevant to the customer, such as local environmental or other regulations.....

    automateandvalidate

    BalasHapus
  37. Very informative blog. Thanks for sharing. Best of Luck to you.

    BalasHapus
  38. Buy Remi Walk In Stability Chamber in India. Get best and cheap prices with installation guide. Know the features, application, technical specification, certificates and product comparison for Remi Walk In Stability Chamber

    Walk In Stability Chamber|| Laboratory Stability Chamber

    BalasHapus
  39. nice posting,thanks for sharing this information.Magnetic stirrers are always the first choice when you are facing crucial stirring applications in pharmaceutical, laboratory and biomedical research applications.

    Magnetic Stirrer Hot Plate

    BalasHapus
  40. nice posting.Orbital Shaking Incubators are specially designed to have both operation of incubation and shaking. Orbital Shaking Incubators are available with or without refrigeration system to work with different temperature conditions.

    Cooling Incubator||Shaking Incubator

    BalasHapus
  41. When you are looking buy a microbiology lab equipments, first of all you should know your budget because most lab equipments are expensive.
    absorbance cuvette
    http://www.precisioncells.com/

    BalasHapus
    Balasan
    1. Yes, you said it right when you are buying equipments you should have good budget.
      http://mccurdysciencematters.blogspot.in/2013/10/speed-lab-report.html

      Hapus
  42. Buenas Fiestas y Muy Feliz Año 2015

    Saludos afectuosos.-

    BalasHapus
  43. Pengen yang lebih seru ...
    Ayo kunjungi www.asianbet77.com
    Buktikan sendiri ..

    Real Play = Real Money

    - Bonus Promo Red Card pertandingan manapun .
    - Bonus Mixparlay .
    - Bonus Tangkasnet setiap hari .
    - New Produk Sabung Ayam ( minimal bet sangat ringan ) .
    - Referal 5 + 1 % ( seumur hidup ) .
    - Cash Back up to 10 % .
    - Bonus Royalty Rewards setiap bulan .

    Untuk Informasi lebih jelasnya silahkan hubungi CS kami :
    - YM : op1_asianbet77@yahoo.com
    - EMAIL : melasian77cs@gmail.com
    - WHATSAPP : +63 905 213 7234
    - WECHAT : asianbet_77
    - SMS CENTER : +63 905 209 8162
    - PIN BB : 2B4BB06A / 28339A41

    Salam Admin ,
    asianbet77.com

    Download Disini

    BalasHapus
  44. Thanks for sharing your valuable information.I found it very useful.Keep posting amazing content like this.
    Plastic Labware India

    BalasHapus